Treponema denticola associates with increased levels of MMP-8 and MMP-9 in gingival crevicular fluid
Article first published online: 7 JAN 2013
© 2012 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Volume 19, Issue 7, pages 694–701, October 2013
How to Cite
Oral Diseases (2013) 19, 694–701
- Issue published online: 19 AUG 2013
- Article first published online: 7 JAN 2013
- Accepted manuscript online: 8 DEC 2012 02:37AM EST
- Manuscript Accepted: 2 DEC 2012
- Manuscript Revised: 11 OCT 2012
- Manuscript Received: 14 JUN 2012
- Karolinska Institutet, Sweden, Philips Oral Healthcare Inc.
- Academy of Finland and Helsinki University Central Hospital Research Foundation
- Medical Society of Finland
- Treponema denticola ;
- matrix metalloproteinases;
- gingival crevicular fluid
The aim was to assess the association between the presence of site-specific subgingival micro-organisms and the levels of matrix metalloproteinases-8 and matrix metalloproteinases-9 (MMP-8 and MMP-9) in gingival crevicular fluid (GCF).
Materials and Methods
The patient group consisted of 56 subjects with periodontitis and the control group of 43 subjects without periodontitis. GCF samples from four test sites for each subject were collected. Polymerase chain reaction was used to detect the presence of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, and Treponema denticola. MMP-8 concentrations were analyzed by a time-resolved immunofluorometric assay, and MMP-9 levels were determined by enzyme-linked immunosorbent assay. Student's unpaired t-test, chi-square test, and Fisher's exact P-value were calculated.
The presence of T. denticola in the test sites was significantly higher in the patient group than in the control group. The presence of T. forsythia and T. denticola was associated with increased levels of MMP-8 in the test sites. Respectively, site-specific presence of T. denticola was associated with an increase in MMP-9 levels in three of the four test sites.
The presence of subgingival micro-organisms in GCF, particularly T. denticola, appeared to induce a host response with an increased release of MMP-8 and MMP-9 in the test sites.