The association of IL-6 and IL-6R gene polymorphisms with chronic periodontitis in a Chinese population

Authors

  • H-Y Zhang,

    1. Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Science, Lanzhou University, Lanzhou City, Gansu Province, China
    2. Institute of Hematology, Lanzhou General Hospital of PLA, Lanzhou, China
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  • L Feng,

    1. Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Science, Lanzhou University, Lanzhou City, Gansu Province, China
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  • H Wu,

    1. Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Science, Lanzhou University, Lanzhou City, Gansu Province, China
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  • X-D Xie

    Corresponding author
    1. Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Science, Lanzhou University, Lanzhou City, Gansu Province, China
    • Correspondence: Professor Xiao-dong Xie, Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Science, 199 West DongGang Road, Lanzhou University, Lanzhou City, Gansu Province 730000, China. Tel: +86 931 8915023, Fax: +86 931 8915001, E-mail: xdxie@lzu.edu.cn

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Abstract

Objective

The aim of our investigation is to reveal the association of interleukin-6 (IL-6) and interleukin-6 receptor (IL-6R) gene polymorphisms with chronic periodontitis in Northwestern Chinese Han Population.

Material and Methods

A case–control study of 199 cases of chronic periodontitis patients and 216 healthy controls was performed. Genomic DNA was isolated from whole venous blood, and four single nucleotide polymorphisms (IL-6 −572 G/C and −1363 G/T, IL-6R −183 G/A and +48892 A/C) were analysed using polymerase chain reaction, followed by restriction fragment length polymorphism analysis and sequencing methods.

Results

Our SNP analyses showed that the distribution of the IL-6 −572 G/C and IL-6R −183 G/A polymorphisms did not differ between patients and controls. The frequency of the IL-6 −1363 G/T genotype GG was significantly increased chronic periodontitis cases (= 0.023, odds ratio (OR) = 2.825 adjusted for gender and age.) The IL-6R +48892 A/C polymorphisms genotype CC was found to be protective against chronic periodontitis (= 0.004, OR = 0.318 adjusted for gender and age.). In addition, compared with the other haplotypes, haplotype A+48892A−183 was significantly associated with chronic periodontitis, with an odds ratio of 0.720 (= 0.0235).

Conclusion

Our results suggest that the IL-6 −1363 G/T and IL-6R +48892 A/C polymorphisms may contribute to genetic susceptibility to chronic periodontitis in Northwestern Chinese Han population.

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