Evaluation of salivary biomarker profiles following non-surgical management of chronic periodontitis
Article first published online: 18 MAR 2013
© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Volume 20, Issue 2, pages 171–177, March 2014
How to Cite
Oral Diseases (2014) 20, 171–177
- Issue published online: 11 FEB 2014
- Article first published online: 18 MAR 2013
- Accepted manuscript online: 14 FEB 2013 02:49AM EST
- Manuscript Accepted: 7 FEB 2013
- Manuscript Revised: 14 DEC 2012
- Manuscript Received: 22 AUG 2012
- toll-like receptors;
- CD14 antigen;
- biological markers;
- dental scaling;
- root planing;
- chronic periodontitis
Soluble toll-like receptor-2 (sTLR-2) and cytokines in saliva were assessed as clinical markers for chronic periodontitis in a longitudinal study.
Materials and Methods
Unstimulated whole saliva was collected from 20 periodontally healthy individuals and 20 patients with chronic periodontitis at diagnosis and at 1 and 6 weeks following scaling and root planing (SRP). Biomarkers including the cytokines (IL-4, IL-6, IL-10, and IL-17), sTLR-2, and sCD14 in saliva were measured by enzyme-linked immunosorbent assay. Mann–Whitney U-test and Student's t-test were used to determine the significance between healthy and chronic periodontitis groups and that between pre- and post-SRP samples, respectively.
Salivary sTLR-2, IL-17, and IL-10 levels were significantly lower and those of sCD14, IL-6, and IL-4 were significantly higher in patients with chronic periodontitis as compared with healthy controls. Furthermore, sTLR-2 and IL-4 in saliva reached levels comparable to those of healthy individuals at 6-week re-evaluation visit, implicating a correlation of the two markers with the disease process.
Our data suggest that salivary sTLR-2 is a potential prognostic or maintenance marker for chronic periodontitis. The observed variability of salivary cytokines is consistent with the role of these cytokines in the progression of chronic periodontitis.