Cartilage degradation in temporomandibular joint induced by unilateral anterior crossbite prosthesis
Article first published online: 25 APR 2013
© 2013 John Wiley & Sons A/S
Volume 20, Issue 3, pages 301–306, April 2014
How to Cite
Oral Diseases (2014) 20, 301–306
- Issue published online: 14 MAR 2014
- Article first published online: 25 APR 2013
- Accepted manuscript online: 8 APR 2013 07:06AM EST
- Manuscript Accepted: 24 MAR 2013
- Manuscript Revised: 19 FEB 2013
- Manuscript Received: 27 NOV 2012
- National Natural Science Foundation of China. Grant Number: 81271169
- temporomandibular joint;
- temporomandibular disorder
To investigate the pathological effects on temporomandibular joint cartilage of an experimentally created unilateral anterior crossbite prosthesis.
Six-week-old female Sprague–Dawley rats were divided into experimental and control groups and further divided into three subgroups for three time points (n = 9). In experimental groups, the unilateral anterior crossbite was created by bonding two metal tubes on left maxillary and mandibular incisors. 2, 4, or 8 weeks after operation, the histomorphological changes and the expression changes of PCNA, COL II, aggrecan, MMP-3, MMP-9, MMP-13, and TIMP-1 in condylar cartilage were investigated by hematoxylin–eosin and toluidine blue staining, real-time PCR, and immunohistochemistry.
In experimental groups, decreasing in chondrocytes, cell-free areas, reduction in the thickness of hypertrophic layer, and loss of extracellular matrix were noticed. The expression level of PCNA was decreased at week 2 and week 4. The mRNA expression of COL II was decreased at 4 week and that of aggrecan was increased at 2 week but decreased at 4 and 8 week. The expression of MMP-9, MMP-13, and TIMP-1 was increased at 2-week and decreased at 4- and 8-week, while that of MMP-3 was increased at 4- and 8-week.
The present unilateral anterior crossbite prosthesis led to degradation of mandibular condylar cartilage.