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Ovariectomy induces osteoporosis in the maxillary alveolar bone: an in vivo micro-CT and histomorphometric analysis in rats

Authors

  • Q-G Dai,

    1. Department of Oral and Cranio-maxillofacial Science, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    2. Shanghai Key Laboratory of Stomatology, Shanghai, China
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  • P Zhang,

    1. Department of Oral and Cranio-maxillofacial Science, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    2. Shanghai Key Laboratory of Stomatology, Shanghai, China
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  • Y-Q Wu,

    1. Department of Oral and Cranio-maxillofacial Science, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    2. Shanghai Key Laboratory of Stomatology, Shanghai, China
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  • X-H Ma,

    1. Department of Oral and Cranio-maxillofacial Science, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    2. Shanghai Key Laboratory of Stomatology, Shanghai, China
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  • J Pang,

    1. Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China
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  • L-Y Jiang,

    Corresponding author
    1. Department of Oral and Cranio-maxillofacial Science, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    2. Shanghai Key Laboratory of Stomatology, Shanghai, China
    • Correspondence: Ling-Yong Jiang, DDS, PhD and Bing Fang, DDS, PhD, Department of Oral and Cranio-maxillofacial Science, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University and Shanghai Key Laboratory of Stomatology, Room 405, Building 1, No. 639 Zhi Zao Ju Road, Shanghai 200011, China. Tel: +86 2123272699 ext. 5735, Fax: +86 21 63136856, E-mails: jly117@sina.com and fangbing@sjtu.edu.cn

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  • B Fang

    Corresponding author
    1. Department of Oral and Cranio-maxillofacial Science, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    2. Shanghai Key Laboratory of Stomatology, Shanghai, China
    • Correspondence: Ling-Yong Jiang, DDS, PhD and Bing Fang, DDS, PhD, Department of Oral and Cranio-maxillofacial Science, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University and Shanghai Key Laboratory of Stomatology, Room 405, Building 1, No. 639 Zhi Zao Ju Road, Shanghai 200011, China. Tel: +86 2123272699 ext. 5735, Fax: +86 21 63136856, E-mails: jly117@sina.com and fangbing@sjtu.edu.cn

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Abstract

Objective

To investigate whether porotic changes occur in maxillary alveolar bone in ovariectomized rats.

Methods

Thirty-two 6-week-old female Sprague-Dawley rats were divided into an ovariectomy group (OVX) and a sham-ovariectomy group (sham). Twelve weeks after surgery, maxillae of 16 rats (eight OVX and eight sham) were analyzed by micro-CT. Inter-radicular alveolar bone of the maxillary first molar was reconstructed and analyzed. The remaining 16 maxillae were used for histochemistry, including hematoxylin-eosin staining and tartrate-resistant acid phosphatase enzyme staining.

Results

Three-dimensional reconstructed images of the irregular alveolar bone showed an intuitive view of porotic changes in the OVX group. The alveolar bone in OVX rats had a porous microarchitecture including lower bone mass and a looser structure of more widely separated trabeculae. Bone mineral density (BMD), bone volume fraction (BV/TV), and trabecular thickness (Tb.Th.) were significantly lower in the OVX group than those in the sham group (< 0.05). Trabecular separation (Tb.Sp.) increased significantly in the OVX rats compared to the sham rats (< 0.05). Histomorphometric analysis of alveolar bone also revealed porotic changes in the OVX rats. Bone area ratio significantly decreased in the OVX group compared with the sham group (< 0.01). There were also more osteoclasts present in the alveolar bone of OVX rats compared to sham rats (< 0.05).

Conclusion

Ovariectomy induces osteoporosis in maxillary alveolar bone in rats, which may be related to the increased number of osteoclasts.

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