Harmine promotes periodontal ligament cell-induced tissue regeneration

Authors

  • Hyun-Chang Lim,

    1. Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea
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  • Byung-Yoon Cha,

    1. Research Institute for Biological Functions, CHUBU University, Aichi, Japan
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  • Sun U. Song,

    1. Clinical Research Center, School of Medicine, Inha University, Incheon, Republic of Korea
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  • Jeong-Ho Yun

    Corresponding author
    1. Department of Periodontology, School of Dentistry and Institute of Oral Bioscience, Chonbuk National University, Jeonju, Republic of Korea
    2. Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea
    • Corresponding author:

      Jeong-Ho Yun

      Department of Periodontology, School of Dentistry, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, 54896 Republic of Korea

      Tel.: +82-63-2502289

      Fax: +82-63-2502289

      E-mail:grayheron@hanmail.net

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  • This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1111/odi.12770

Abstract

Objective

to investigate whether harmine has a promotive effect on human periodontal ligament cells (hPDLCs)-induced tissue regeneration.

Materials and Methods

Various concentrations of harmine on hPDLCs proliferation were tested. Osteogenic and cementogenic characteristics were examined in hPDLC/rhBMP-2 and hPDLC/harmine by alizarin red S staining, real-time PCR, and Western blotting assay. The activity of harmine was investigated in an ectopic transplantation nude mouse model.

Results

We determined that 10 μM of harmine was the threshold concentration. hPDLC/harmine showed similar mineralized nodule formation in alizarin S staining compared to hPDLC/rhBMP-2. In real-time PCR, a highest gene expression level was observed for Runx2 in hPDLC/harmine at all time points. The level of CEMP-1 in hPDLC/harmine was higher at 7days than hPDLCs alone. Thicker band of Runx2 in hPDLC/harmine was observed than in hPDLC/rhBMP-2 at 7 days by Western blotting. The band for CEMP-1 in hPDLC/harmine was thicker than hPDLCs alone at both 7 and 14 days. In ectopic transplantation, hPDLCs with harmine showed a comparable amount of mineralized tissue formation compared to rhBMP-2. hPDLCs with harmine or rhBMP-2 formed both bone and cementum-like tissue with Sharpey's fiber-like collagen insertion.

Conclusion

Harmine can be a potential candidate for promoting hPDLCs-induced tissue regeneration.

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