Children monosensitized to pine nuts have similar patterns of sensitization
Article first published online: 29 OCT 2012
© 2012 John Wiley & Sons A/S
Pediatric Allergy and Immunology
Volume 23, Issue 8, pages 761–764, December 2012
How to Cite
To cite this article: Children monosensitized to pine nuts have similar patterns of sensitization. Pediatr Allergy Immunol 2012: 00: 000–000., , , , , , , , .
- Issue published online: 29 NOV 2012
- Article first published online: 29 OCT 2012
- Manuscript Accepted: 6 SEP 2012
- University of Florence
- A. Meyer Children Hospital
- pine nuts allergy
Several cases of pine nut allergies and anaphylaxis have been reported in the literature, but only few pine nut allergens have been characterized. The aim of this research is to identify through immunoelectrophoretic techniques the major pine nut allergens in a group of children monosensitized to pine nuts.
We studied five children with pine nut allergies and no other sensitization to food except to pine nuts, confirmed by in vivo (prick test, prick-to-prick) and in vitro tests (specific IgE determinations [CAP-FEIA]). The protein profile of pine nuts was analyzed by Sodium Dodecyl sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Immunoblotting was performed after incubation of membranes with the sera from the children included in the present study.
Immunoblotting (SDS-PAGE) demonstrated five similar bands between 6 and 47 kDa in all the subjects studied.
These bands should be considered the potential allergens for pine nut allergic children.