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Keywords:

  • basophil activation;
  • CD63;
  • egg allergy;
  • specific oral tolerance induction

Abstract

Background

In the last decade, there have been an increasing number of studies on achieving tolerance to foods by specific oral tolerance induction (SOTI). Still, the underlying mechanism of SOTI is unknown. Our aim was to describe changes in CD63 expression on basophils following in vitro Ag-specific stimulation by basophil activation test (BAT), after SOTI with egg in a pediatric population.

Methods

Ten children with persistent allergy to egg were included. Skin prick tests (SPTs) and open food challenges (OFCs) were performed before SOTI. Specific IgE determination and BAT with egg white (EW), ovomucoid (OM), and ovalbumin (OVA) were performed before and after 1 month of the buildup phase of SOTI.

Results

Total tolerance to egg was achieved in 9 cases and partial in one.

After SOTI, there was a significant decrease in mean specific IgE levels (p < 0.05). CD63 expression also decreased (p < 0.05) in all patients.

Conclusion

Decrease in Ag-specific basophil responsiveness is associated with the development of clinical tolerance by SOTI.