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Keywords:

  • fluid therapy;
  • crystalloid solutions;
  • infusions;
  • intravenous;
  • blood coagulation;
  • blood coagulation disorders;
  • thrombelastography

Summary

Background and Objectives

Impairment of blood coagulation is one of the main side effects of volume replacement, particularly if artificial colloids such as hydroxyethyl starch (HES) and gelatine preparations are used. This animal study aimed to evaluate the effect of a single fast intravenous crystalloid or colloid fluid bolus on blood coagulation as measured by rotation thromboelastometry (ROTEM®).

Methods

Thirty-two anesthetized piglets were infused with a rapid 20 ml·kg−1 fluid bolus of either normal saline (NS), 4% gelatine, 5% albumin or 6% HES 130/0.4 (n = 8 per group) over a period of 2 min. Hemostasis was assessed by ROTEM® before and 1 min after fluid administration. Within-group differences were analyzed by Wilcoxon test, and additionally overall Kruskal–Wallis test followed by posthoc Mann–Whitney U-test were applied to detect differences between groups.

Results

All fluids caused a significant weakening of clot strength within groups. HES and gelatine showed a significantly stronger impairment of clot growth and maximum clot firmness as compared with albumin and normal saline. Impairment of fibrin polymerization was more pronounced following HES as compared with all other fluids.

Conclusion

After moderate but very fast volume loading, HES and gelatine impair blood coagulation to a larger extent as compared with albumin or normal saline, while no significant differences were observed between both artificial colloids.