• Open Access

Increased expression of phospholipase Dα1 in guard cells decreases water loss with improved seed production under drought in Brassica napus

Authors

  • Shaoping Lu,

    1. National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
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  • Sung C. Bahn,

    1. Department of Biology, University of Missouri, St. Louis, MO, USA
    2. Donald Danforth Plant Science Center, St. Louis, MO, USA
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  • Gang Qu,

    1. National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
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  • Haiying Qin,

    1. National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
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  • Yue Hong,

    1. National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
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  • Qingpeng Xu,

    1. National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
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  • Yongming Zhou,

    1. National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
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  • Yueyun Hong,

    Corresponding author
    • National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
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  • Xuemin Wang

    Corresponding author
    1. Department of Biology, University of Missouri, St. Louis, MO, USA
    2. Donald Danforth Plant Science Center, St. Louis, MO, USA
    • National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China
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Correspondence (fax (314) 587 1519; email swang@danforthcenter.org; hongyhk@yahoo.com.cn)

Summary

The activation of phospholipase Dα1 (PLDα1) produces lipid messenger phosphatidic acid and promotes stomatal closure in Arabidopsis. To explore the use of the PLDα1-mediated signalling towards decreasing water loss in crop plants, we introduced Arabidopsis PLDα1 under the control of a guard cell–specific promoter AtKatIpro into two canola (Brassica napus) cultivars. Multiple AtKatIpro::PLDα1 lines in each cultivar displayed decreased water loss and improved biomass accumulation under hyperosmotic stress conditions, including drought and high salinity. Moreover, AtKatIpro::PLDα1 plants produced more seeds than did WT plants in fields under drought. The results indicate that the guard cell–specific expression of PLDα1 has the potential to improve crop yield by enhancing drought tolerance.

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