• Open Access

Altered expression of the PTR/NRT1 homologue OsPTR9 affects nitrogen utilization efficiency, growth and grain yield in rice

Authors

  • Zhongming Fang,

    1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
    2. University of Chinese Academy of Sciences, Beijing, China
    Current affiliation:
    1. Wuhan Bioengineering Institute, Wuhan, China
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  • Kuaifei Xia,

    1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
    2. Key Laboratory of South China Agricultural Plant Genetics and Breeding, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
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  • Xin Yang,

    1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
    2. University of Chinese Academy of Sciences, Beijing, China
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  • Marianne Suter Grotemeyer,

    1. Institute of Plant Sciences, University of Bern, Bern, Switzerland
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  • Stefan Meier,

    1. Institute of Plant Sciences, University of Bern, Bern, Switzerland
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  • Doris Rentsch,

    1. Institute of Plant Sciences, University of Bern, Bern, Switzerland
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  • Xinlan Xu,

    1. Key Laboratory of South China Agricultural Plant Genetics and Breeding, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
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  • Mingyong Zhang

    Corresponding author
    1. Key Laboratory of South China Agricultural Plant Genetics and Breeding, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
    • Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
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  • Accession number: OsPTR9 (LOC_06g49250).

Correspondence (fax +86 20 3725 2979;

email zhangmy@scbg.ac.cn)

Summary

The plant PTR/NRT1 (peptide transporter/nitrate transporter 1) gene family comprises di/tripeptide and low-affinity nitrate transporters; some members also recognize other substrates such as carboxylates, phytohormones (auxin and abscisic acid), or defence compounds (glucosinolates). Little is known about the members of this gene family in rice (Oryza sativa L.). Here, we report the influence of altered OsPTR9 expression on nitrogen utilization efficiency, growth, and grain yield. OsPTR9 expression is regulated by exogenous nitrogen and by the day-night cycle. Elevated expression of OsPTR9 in transgenic rice plants resulted in enhanced ammonium uptake, promotion of lateral root formation and increased grain yield. On the other hand, down-regulation of OsPTR9 in a T-DNA insertion line (osptr9) and in OsPTR9-RNAi rice plants had the opposite effect. These results suggest that OsPTR9 might hold potential for improving nitrogen utilization efficiency and grain yield in rice breeding.

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