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Figure S1 Sequence alignment of native and plant-optimized gag genes and their expected translation product.

Figure S2 Sequence alignment of native and plant-optimized dgp41 genes and their expected translation product.

Figure S3 Codon usage of plant-optimized gag and dgp41 is comparable with that of a highly expressed plant gene.

Figure S4 Protein sequences homologues to NMT-1 found in humans, Arabidopsis thaliana, and rice were aligned using the T-Coffee programme, and the degree of similarity is shown as a heat diagram superimposed on the sequences.

Figure S5 Protein sequences related to Tsg101 found in humans, Arabidopsis, and rice were aligned using the T-Coffee programme, and the degree of similarity is shown as a heat diagram superimposed on the sequences.

Figure S6 Protein sequences related to Alix found in humans, Arabidopsis and rice were aligned using the T-Coffee programme, and the degree of similarity is shown as a heat diagram superimposed on the sequences.

Appendix S1 Biological and biochemical characterization of HIV-1 Gag/dgp41 virus-like particles expressed in Nicotiana benthamiana.

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