Accession numbers (GenBank/EMBL): Sequence data from this article can be found in the following accession numbers: ACT2 (At3g18780, http://www.ncbi.nlm.nih.gov/nuccore?term=At3g18780), ACTINNT (GQ339768, http://www.ncbi.nlm.nih.gov/nuccore/GQ339768) and PTXD (AF061070, http://www.ncbi.nlm.nih.gov/nuccore/AF061070).
A novel dominant selectable system for the selection of transgenic plants under in vitro and greenhouse conditions based on phosphite metabolism
Article first published online: 27 MAR 2013
© 2013 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 11, Issue 4, pages 516–525, May 2013
How to Cite
2013) A novel dominant selectable system for the selection of transgenic plants under in vitro and greenhouse conditions based on phosphite metabolism. Plant Biotechnol J doi: 10.1111/pbi.12063, (
- Issue published online: 25 APR 2013
- Article first published online: 27 MAR 2013
- Manuscript Accepted: 9 FEB 2013
- Manuscript Revised: 4 FEB 2013
- Manuscript Received: 11 DEC 2012
- Howard Hughes Medical Institute. Grant Number: Grant 55005946
Figure S1 Expression levels of ptxD gene in Arabidopsis transgenic lines.
Figure S2 Evaluation of the sensitivity of Arabidopsis transgenic plants to phosphite on liquid media.
Figure S3 Schematic representation of the construct designed to express the ptxD gene under control of CaMV 35S promoter.
Figure S4 Selection of Arabidopsis transformants using the ptxD gene as a selectable marker and different concentrations of Phi as selectable agent.
Figure S5 Amplification of the ptxD gene in T1 Arabidopsis thaliana transformants.
Figure S6 Phenotype of transgenic tobacco plants.vFigure S7 Presence of the ptxD gene in T1 tobacco transformants.
Figure S8 Selection of Arabidopsis and tobacco transformants under greenhouse conditions using the ptxD/Phi system.
Table S1 The T2 progeny of Arabidopsis independent lines, selected using the ptxD/Phi system, was sown and germinated in Phi-containing media.
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