Marker-free genetic engineering of the chloroplast in the green microalga Chlamydomonas reinhardtii
Article first published online: 7 MAY 2013
© 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd
Plant Biotechnology Journal
Volume 11, Issue 7, pages 818–828, September 2013
How to Cite
2013) Marker-free genetic engineering of the chloroplast in the green microalga Chlamydomonas reinhardtii. Plant Biotechnol. J. doi: 10.1111/pbi.12073, (
- Issue published online: 20 AUG 2013
- Article first published online: 7 MAY 2013
- Manuscript Accepted: 20 MAR 2013
- Manuscript Revised: 5 MAR 2013
- Manuscript Received: 18 DEC 2012
|pbi12073-sup-0001-FigS1.pdf||application/PDF||59K||Figure S1 Sequence alignment of ADH1 gene from Saccharomyces cerevisiae (ScADH1) and the Chlamydomonas reinhardtii chloroplast codon optimized ADH1 gene (CrCpADH1).|
Figure S2 Expression of rbcL90-ADH1 and ADH1 proteins in E. coli.
Figure S3 Ethanol production measurements.
|pbi12073-sup-0003-TextS1.doc||Word document||53K||Data S1 Supplementary experimental procedures for Strains, culture media, and growth conditions; Extraction of genomic DNA; Expression vector for Chlamydomonas reinhardtii chloroplast transformation; Generation of CrCpADH1 specific polyclonal antibodies; Protein analysis by SDS-PAGE and Western blotting; Ethanol production measurements.|
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