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Figure S1 (A) Amino acid sequence alignment of MdEPSPS and others EPSPSs [AtEPSPS (NP_182055), AmEPSPS (ABE77393), ArEPSPS (AAT45234), BnEPSPS (CAA35839), CaEPSPS (AA V64030), CcEPSPS (AAT45243), DcEPSPS (AAL27698), DsEPSPS (ABM68632), EiEPSPS (CAD01096), EaEPSPS (AAT45236), LmEPSPS (AAZ79230), MtEPSPS (ABE85250), NtEPSPS (AAA34071), OvEPSPS (AAL65913), OsEPSPS (AAL07437), PvEPSPS (ABG88197), SlEPSPS (P10748), ZmEPSPS, BcEPSPS (EAY62485), YbEPSPS (ZP_00822355)]. (B) Phylogenetic tree analysis of MdEPSPS using MEGA. Others EPSPSs shown in this Fig are from: [AtEPSPS (NP_182055), AmEPSPS (ABE77393), ArEPSPS (AAT45234), BnEPSPS (CAA35839), CaEPSPS (AAV64030), CcEPSPS (AAT45243), DcEPSPS (AAL27698), DsEPSPS (ABM68632), EiEPSPS (CAD01096), EaEPSPS (AAT45236), LmEPSPS (AAZ79230), MtEPSPS (ABE85250), NtEPSPS (AAA34071), OvEPSPS (AAL65913), OsEPSPS (AAL07437), PvEPSPS (ABG88197), SlEPSPS (P10748), ZmEPSPS, BcEPSPS (EA Y62485), YbEPSPS (ZP_00822355)].

Figure S2 Comparative image of root length of transgenic Arabidopsis seeds on an MS medium containing various glyphosate concentrations (0, 200, 750, and 1000 μm) in Petri dishes.

Figure S3 Location of the two mutated amino acid residues in the three-dimensional structure of MdEPSPSmutant.

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