A method for rapid production of heteromultimeric protein complexes in plants: assembly of protective bluetongue virus-like particles
Article first published online: 6 MAY 2013
© 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd
Plant Biotechnology Journal
Volume 11, Issue 7, pages 839–846, September 2013
How to Cite
2013) A method for rapid production of heteromultimeric protein complexes in plants: assembly of protective bluetongue virus-like particles. Plant Biotechnol. J. doi: 10.1111/pbi.12076, , , , (
- Issue published online: 20 AUG 2013
- Article first published online: 6 MAY 2013
- Manuscript Accepted: 31 MAR 2013
- Manuscript Revised: 14 MAR 2013
- Manuscript Received: 30 OCT 2012
- Marie Curie Early Stage Training Fellowship. Grant Number: MEST-CT-2005-019727
- Trustees of the John Innes Foundation
- PLAPROVA. Grant Number: KBBE-2008-227056
- John Innes Centre. Grant Number: BB/J004561/1
- John Innes Foundation
Figure S1 Some BTV8 structural proteins cause symptoms when expressed in plants individually, but not when expressed in combination with their structural neighbouring proteins.
Figure S2 Mass spectrometry analysis of 4 main protein bands recovered from SDS-PAGE analysis of a density gradient fractions from leaf tissue coinfiltrated with constructs for coexpression of BTV-8 VP2, VP3, VP5 and VP7.
Figure S3 Clarified extract from leaf tissue expression four BTV structural proteins, separated on two consecutive step sucrose gradients.
Figure S4 Densitometric analysis of Coomassie-stained gel of Figure 3b (inset).
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