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Figure S1 Phenotypes of two additional barley lines expressing the heterologous Lr34res, resulting in an extreme LTN phenotype.

Figure S2 The LTN phenotype is indicative for the insertion of the full-length genomic Lr34res transgene.

Figure S3 Representative examples of pathogen infections on transgenic barley lines not presented in Fig. 2.

Figure S4 Analysis of transgenic cLr34res (Bc and Bc-GFP) lines and genomic Lr34sus (BGS) lines.

Figure S5 DNA copy number of the transgenic event BG8 transformed with p6U:gLr34res.

Figure S6 Analysis of HvS40 and Lr34res transcript levels in seedlings exhibiting the LTN phenotype.

Table S1 Measurements of plant vigour and total grain weight for transgenic barley lines and their controls grown under standard glass house conditions (SGC) or at 13 °C.

Table S2 Primers used throughout the production and subsequent characterization of transgenic lines.

Table S3 qPCR primers used for target gene transcript level analysis in transgenic lines.

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