pbi12078-sup-0001-FigS1.tifimage/tif5846KFigure S1 Regulatory model of aliphatic glucosinolate (GSL) biosynthetic pathway based on literature available in Arabidopsis (Gigolashvili et al., 2007; Halkier and Gershenzon, 2006; Hirai et al., 2007; Sønderby et al., 2010b).
pbi12078-sup-0002-FigS2.tifimage/tif8777KFigure S2 Sequence alignment of BjMYB28 and BjMYB29 proteins.
pbi12078-sup-0003-FigS3.tifimage/tif1111KFigure S3 PCR amplification of BjMYB28-3 transcript across developmental stages of Brassica juncea lines.
pbi12078-sup-0004-FigS4.tifimage/tif792KFigure S4 Comparison of expression patterns of BjMYB28 and BjMYB29 gene homologs across developmental stages of high glucosinolate Brassica juncea cv. Varuna.
pbi12078-sup-0005-FigS5.tifimage/tif1772KFigure S5 Southern blot analysis of BjMYB28-3 gene across glucosinolate contrasting cultivars of Brassica juncea.
pbi12078-sup-0006-FigS6.tifimage/tif6603KFigure S6 Nucleotide sequence alignment of BjMYB28 and BjMYB29 homologs at RNAi target region.
pbi12078-sup-0007-FigS7.tifimage/tif885KFigure S7 qRT-PCR analysis of BjMYB28-3 and BjMYB29-1 transcripts in pods of low glucosinolate transgenic lines.
pbi12078-sup-0008-TableS1-S5.docWord document178K

Table S1 Leaf and seed glucosinolate content and profiles (in μmol g-1 DW) across glucosinolate contrasting lines of Brassica juncea.

Table S2 Total glucosinolate content (μmol/g DW) in T1 seeds of transgenic events. The total GSL was estimated using the Near Infrared Reflectance Spectroscopy.

Table S3 Total seed GSL content among T1 progeny derived from single-copy BjMYB28(RNAi) transgenic events.

Table S4. Mean aliphatic, indolic and total glucosinolate contents (µmol/g DW) in the leaves of low glucosinolate single-copy homozygous (T3) transgenic lines.

Table S5 List of primers used in the current study.

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