SEARCH

SEARCH BY CITATION

FilenameFormatSizeDescription
pbi12079-sup-0001-Tables-Figures.docxWord document1251K

Figure S1 Half-normal plot showing the strongest factors and their significant interactions in the second nutrient screen

Figure S2 Interaction plot matrix from the second nutrient screen with four strong interactions: NH4NO3/CaCl2, CaCl2/MgSO4, NH4NO3/KI and KI/MnSO4.

Figure S3 Factors plot from the phytohormone screen. The auxins IBA and 2,4-D exerted the most potent effect.

Figure S4 Interactions identified during the phytohormone/regulators screen: (a) Auxins with cytokinins. (b) Auxins with GA3. (c) Auxins with MeJa.

Figure S5 Important two-factor interactions in the combined screen design. (a) Auxins with cytokinins. (b) Auxins with KNO3. (c) Auxins with NH4NO3. (d) Auxins with CaCl2.

Figure S6 Plot of the significant CaCl2 × NH4NO3 × 2,4-D+ interaction in the combined screen design. The most favourable combination was CaCl2 (5.98 mm), NH4NO3 (5.49 mm) and 2,4-D+ (0.9 μm).

Table S1 Low and high concentration levels of screened macronutrient and micronutrient factors.

Table S2 Fractional factorial design for the 13 independent variables in coded values and the M12 concentration in the culture supernatant as measured responses.

Table S3 ANOVA table of the first screening of macronutrient and micronutrient factors.

Table S4 Low and high concentrations levels of the factors in the second nutrient screen design.

Table S5 Fractional factorial design for the six independent variables in coded values and the M12 concentration in the culture supernatant as measured responses, during the second nutrient screen design.

Table S6 ANOVA table of the second screen of macronutrient and micronutrient factors.

Table S7 Low and high concentrations levels of the screened phytohormones and plant regulators.

Table S8 Fractional factorial design of the phytohormones and plant regulators screen, and the M12 concentration in the culture supernatant as the measured responses.

Table S9 ANOVA table of the phytohormones and plant regulators screening.

Table S10 Low and high concentrations levels of the screened factors in the combined design.

Table S11 Fractional factorial design in coded values and the M12 concentration in the culture supernatant, as measured responses during the combined screen design.

Table S12 ANOVA table of the combined screening design.

Table S13 Gradient experiment.

Table S14 Concentration levels in the RSM design.

Table S15 RSM design.

Table S16 The ANOVA table of the RSM design.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.