Metabolic engineering of β-carotene in orange fruit increases its in vivo antioxidant properties
Article first published online: 27 AUG 2013
© 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd
Plant Biotechnology Journal
Volume 12, Issue 1, pages 17–27, January 2014
How to Cite
2013) Metabolic engineering of β-carotene in orange fruit increases its in vivo antioxidant properties. Plant Biotechnol. J., doi: 10.1111/pbi.12112, , , , , , , and (
- Issue published online: 26 DEC 2013
- Article first published online: 27 AUG 2013
- Manuscript Accepted: 23 JUL 2013
- Manuscript Revised: 22 JUL 2013
- Manuscript Received: 8 MAY 2013
- Fondo Europeo de Desarrollo Regional. Grant Numbers: AGL2009-08052, AGL2012-33429
- Ministry of Science and Innovation of Spain
- Generalitat Valenciana
- Citrus Research and Development Foundation from Florida
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Figure S1 Carotenoid biosynthesis in sweet oranges.
Figure S2 Southern blot analysis of Pineapple sweet orange plants transformed with the β-CHX RNAi silencing plasmid.
Figure S3 Effect of heat treatment (90 °C) of the wild type (WT) pulp extracts on microbial contamination, carotenoid profile, and vitamin C content.
Table S1 Carotenoid levels (ng/mL) and vitamin C content (mg AA/100 mL) in the pulp extracts used for nematode diet supplementation.
Data S1 Supplemental experimental procedures (a) Plasmid construction. (b) Southern blot analysis. (c) Processing and biochemical analysis of pulp extracts/worm food. (d) Supplemental references.
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