Reduced generation time of apple seedlings to within a year by means of a plant virus vector: a new plant-breeding technique with no transmission of genetic modification to the next generation
Article first published online: 3 SEP 2013
© 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd
Plant Biotechnology Journal
Volume 12, Issue 1, pages 60–68, January 2014
How to Cite
2013) Reduced generation time of apple seedlings to within a year by means of a plant virus vector: a new plant-breeding technique with no transmission of genetic modification to the next generation. Plant Biotechnol. J., doi: 10.1111/pbi.12116, , (
- Issue published online: 26 DEC 2013
- Article first published online: 3 SEP 2013
- Manuscript Accepted: 11 JUL 2013
- Manuscript Revised: 8 JUL 2013
- Manuscript Received: 30 MAY 2013
- Ministry of Agriculture, Forestry and Fisheries
Figure S1 Promotion of flowering in A. thaliana and tobacco plants infected with the ALSV vector expressing FT orthologue genes presented in Table S1.
Figure S2 (a) RT-PCR analysis of apple seedlings infected with ALSV-AtFT/MdTFL1 at 6 mpi MdTFL1 was detected in all 17 samples, but AtFT was not detected in 7 samples No. 2, 3, 6, 9, 11, 19, or 21, indicating that MdTFL1 was stably maintained, while AtFT was deleted in 40% of samples. (b) Apple seedlings infected with ALSV-AtFT/MdTFL1. Sample No. 9, which lost AtFT, shows vegetative growth of the shoot (11 mpi). In contrast, sample No. A1 shows continuous flowering (7.5 mpi).
|pbi12116-sup-0002-TableS1.docx||Word document||15K||Table S1 Effect of ALSV vectors expressing various FT orthologues on the promotion of flowering in A. thaliana and tobacco plants.|
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