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pbi12118-sup-0001-FigS1.tifimage/tif607KFigure S1 Phytochemical analysis of the methanolic extracts of leaves of different transgenic lines. Compounds were quantified by separating methanolic leaf extracts of WT and transgenic lines using HPLC. The graph shows values ± SD of three leaves from each of the independent transgenic line.
pbi12118-sup-0002-FigS2.tifimage/tif866KFigure S2 HPLC profiles illustrating the accumulation of genistein and other flavonoids in transgenic and WT tobacco acid-hydrolysed extracts. (A) Standards: rutin (1), quercetin (2), genistein (3) and kaempferol (4). (B) WT tobacco leaf. (C) GmIFS1-expressing tobacco leaf. (D) AtMYB12-expressing tobacco leaf. (E) AtMYB12- and GmIFS1-co-expressing tobacco leaf.
pbi12118-sup-0003-FigS3.tifimage/tif913KFigure S3 Negative-ion ESI-MS spectrum of standard genistein (A) and genistein of leaf extract (B) from transgenic plant co-expressing AtMYB12 and GmIFS1.
pbi12118-sup-0004-FigS4.tifimage/tif2402KFigure S4 Leaf extract from transgenic plant has oestrogenicity. Transverse sections of uterus (5 μm) were stained with H&E and representative images (40 × magnifications) of different experimental groups are shown. Quantification of data is provided in Table 2.
pbi12118-sup-0005-TableS1.docWord document46KTable S1 List of oligonucleotides and their use in the study.

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