[Correction added on 16 September 2014, after first publication: author's affiliation has been amended.]
Synthetic TAL effectors for targeted enhancement of transgene expression in plants
Version of Record online: 23 DEC 2013
© 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd
Plant Biotechnology Journal
Volume 12, Issue 4, pages 436–446, May 2014
How to Cite
Liu, W., Rudis, M. R., Peng, Y., Mazarei, M., Millwood, R. J., Yang, J.-P., Xu, W., Chesnut, J. D. and Stewart, C. N. (2014), Synthetic TAL effectors for targeted enhancement of transgene expression in plants. Plant Biotechnology Journal, 12: 436–446. doi: 10.1111/pbi.12150
- Issue online: 18 APR 2014
- Version of Record online: 23 DEC 2013
- Manuscript Accepted: 2 NOV 2013
- Manuscript Revised: 19 OCT 2013
- Manuscript Received: 23 JAN 2013
Figure S1 Effect of Agrobacterium titre on T218-VP64-activated expression of the pporRFP reporter in stable transgenic tobacco plants harbouring 35S::pporRFP (a) or B4 × PR1A::pporRFP (b) as measured by orange fluorescence.
Figure S2 Effects of the C-terminal domain and activation domain of synthetic T118, T218 or T318 on expression of the pporRFP reporter in stable transgenic tobacco plants harbouring 35S::pporRFP at time point 10 days post-inoculation (dpi).
Figure S3 Visual images (representative) of activation of pporRFP expression in stable transgenic tobacco harbouring 35S::pporRFP by additive effects of synthetic TALEs.
Figure S4 The efficiency of Agrobacterium-mediated co-delivery of two TALEs measured by real-time PCR at 96 hpi.
Figure S5 Visual images (representative) of AtPAP1 expression in stable transgenic tobacco harbouring B4 × PR1A::PAP1 by either 4 mm salicylic acid treatment (a; shown in the black outline) or bacterial pathogen Pseudomonas syringae pv. tabaci infection at OD600 = 0.03 (b; shown in the black outline) at 72 hpi.
Table S1 Optimized primer sequences and reaction conditions used for real-time PCR.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.