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Figure S1 Effect of Agrobacterium titre on T218-VP64-activated expression of the pporRFP reporter in stable transgenic tobacco plants harbouring 35S::pporRFP (a) or B4 × PR1A::pporRFP (b) as measured by orange fluorescence.

Figure S2 Effects of the C-terminal domain and activation domain of synthetic T118, T218 or T318 on expression of the pporRFP reporter in stable transgenic tobacco plants harbouring 35S::pporRFP at time point 10 days post-inoculation (dpi).

Figure S3 Visual images (representative) of activation of pporRFP expression in stable transgenic tobacco harbouring 35S::pporRFP by additive effects of synthetic TALEs.

Figure S4 The efficiency of Agrobacterium-mediated co-delivery of two TALEs measured by real-time PCR at 96 hpi.

Figure S5 Visual images (representative) of AtPAP1 expression in stable transgenic tobacco harbouring B4 × PR1A::PAP1 by either 4 mm salicylic acid treatment (a; shown in the black outline) or bacterial pathogen Pseudomonas syringae pv. tabaci infection at OD600 = 0.03 (b; shown in the black outline) at 72 hpi.

Table S1 Optimized primer sequences and reaction conditions used for real-time PCR.

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