OsARF16, a transcription factor, is required for auxin and phosphate starvation response in rice (Oryza sativa L.)

Authors

  • CHENJIA SHEN,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058
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    • These authors contributed equally to this work.

  • SUIKANG WANG,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058
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    • These authors contributed equally to this work.

  • SAINA ZHANG,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058
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  • YANXIA XU,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058
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  • QIAN QIAN,

    1. State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, 359 Tiyuchang Road, Hangzhou, China
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  • YANHUA QI,

    Corresponding author
    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058
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  • DE AN JIANG

    Corresponding author
    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058
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Y. Qi. Fax +86 571 88981355; e-mail qyhjp@zju.edu.cn. D. A. Jiang. Fax +86 571 88206461; e-mail dajiang@zju.edu.cn

ABSTRACT

Plant responses to auxin and phosphate (Pi) starvation are closely linked. However, the underlying mechanisms connecting auxin to phosphate starvation (−Pi) responses are largely unclear. Here, we show that OsARF16, an auxin response factor, functions in both auxin and −Pi responses in rice (Oryza sativa L.). The knockout of OsARF16 led to primary roots (PR), lateral roots (LR) and root hair losing sensitivity to auxin and −Pi response. OsARF16 expression and OsARF16::GUS staining in PR and LR of rice Nipponbare (NIP) were induced by indole acetic acid and −Pi treatments. In −Pi conditions, the shoot biomass of osarf16 was slightly reduced, and neither root growth nor iron content was induced, indicating that the knockout of OsARF16 led to loss of response to Pi deficiency in rice. Six phosphate starvation-induced genes (PSIs) were less induced by −Pi in osarf16 and these trends were similar to a knockdown mutant of OsPHR2 or AtPHR1, which was a key regulator under −Pi. These data first reveal the biological function of OsARF16, provide novel evidence of a linkage between auxin and −Pi responses and facilitate the development of new strategies for the efficient utilization of Pi in rice.

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