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Figure S1. UHPLC-QTOF-MS/MS fragmentation pattern in ESI negative ionization mode for three benzoxazinones.

Figure S2. Multiple reaction monitoring transition table used for nano-infusion-MS/MS quantification and structures of monitored standards. Abbreviation: CT: p- coumaroyltyramine.

Figure S3. Relative fold changes of 11 leaf-biomarkers detected in the roots of leaf-herbivore attacked plants (±SEM). Values have been calculated from the relative mean peak area of each compound (n = 12) and normalized to controls at 24 h.

Appendix S1. Identification of biomarkers by high resolution UHPLC-QTOF-MS/MS and 1HNMR.

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