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Figure S1. Typical laser-scanning confocal microscope image of a tobacco mesophyll cell labeled for mitochondrial O2- with MitoSOX Red. The first panel from left shows the bright-field image, along with air spaces (as). The second panel shows the chlorophyll autofluorescence in blue (false coloration). In the third panel, mitochondrial O2- and the nucleus (marked with an arrow) are shown in red. The last panel is a merged image of all three previous panels. All images are maximum intensity projections of Z-series (8–16 μm in depth). Scale bar = 20 μm.

Figure S2. MnSOD (a), CuZnSOD (b) and FeSOD (c) activity in tobacco leaf at different times post-inoculation with P. syringae pv. phaseolicola. Tobacco lines tested included the WT (closed circle) and two transgenic lines with suppressed levels of AOX (RI9, open triangle; RI29, open square). Data are the mean ± SE of two independent experiments. Data are relative to the SOD activity of the WT at time 0, which was set to 1.

Figure S3. Laser-scanning confocal microscope images of cellular NO in tobacco mesophyll cells at different times post-inoculation with P. syringae pv. phaseolicola. Tobacco lines tested include the WT and two transgenic lines with suppressed levels of AOX (RI9, RI29). All images are maximum intensity projections of Z-series (8–16 μm in depth) and are representative results from three independent experiments, each of which showed similar results. All images are double-labeled with DAF-FM and Mitotracker Red to image both NO (green) and mitochondria (red). Co-localization of these signals is yellow. The control plants (mock-inoculated with H2O) were similar at 4 h and 24 h, so only images taken at 24 h are shown here. Scale bar = 20 μm.

Figure S4. Laser-scanning confocal microscope images of cellular ONOO- in tobacco mesophyll cells at different times post-inoculation with P. syringae pv. phaseolicola. Tobacco lines tested include the WT and two transgenic lines with suppressed levels of AOX (RI9, RI29). All images are maximum intensity projections of Z-series (8–16 μm in depth) and are representative results from three independent experiments, each of which showed similar results. All images are double-labeled with APF and Mitotracker Red to image both ONOO- (green) and mitochondria (red). Co-localization of these signals is yellow. The control plants (mock-inoculated with H2O) were similar at 4 h and 24 h, so only images taken at 24 h are shown here. Scale bar = 20 μm.

FilenameFormatSizeDescription
pce12009_sm_FS1.ppt712KSupporting info item
pce12009_sm_FS2.pptx96KSupporting info item
pce12009_sm_FS3.pptx1715KSupporting info item
pce12009_sm_FS4.pptx1487KSupporting info item

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