Molecular evidence for phytosiderophore-induced improvement of iron nutrition of peanut intercropped with maize in calcareous soil
Version of Record online: 24 APR 2013
© 2013 John Wiley & Sons Ltd
Plant, Cell & Environment
Volume 36, Issue 10, pages 1888–1902, October 2013
How to Cite
XIONG, H., KAKEI, Y., KOBAYASHI, T., GUO, X., NAKAZONO, M., TAKAHASHI, H., NAKANISHI, H., SHEN, H., ZHANG, F., NISHIZAWA, N. K. and ZUO, Y. (2013), Molecular evidence for phytosiderophore-induced improvement of iron nutrition of peanut intercropped with maize in calcareous soil. Plant, Cell & Environment, 36: 1888–1902. doi: 10.1111/pce.12097
- Issue online: 3 SEP 2013
- Version of Record online: 24 APR 2013
- Accepted manuscript online: 18 MAR 2013 03:15AM EST
- Manuscript Accepted: 10 MAR 2013
- Manuscript Revised: 5 MAR 2013
- Manuscript Received: 3 MAY 2012
- National Natural Science Foundation of China. Grant Numbers: 31272223, 31071840
- NSFC. Grant Number: 31121062
Figure S1. Semiquantitative RT-PCR analysis of ZmNAAT1 and ZmDMAS1 in intercropped and monocropped maize roots. Zmtubulin was used as the control gene.
Figure S2. SPAD value of the young leaves in the intercropped and monocropped maize.
Figure S3. Fe concentration in roots and young leaves of intercropped and monocropped peanuts in hydroponics. (a) Fe concentration in roots. (b) Fe concentration in young leaves.
Figure S4. Mass spectrum of DMA by LC-ESI-TOF-MS in intercropped and monocropped peanut roots. (a) DMA and NA peaks in the standard. Peak patterns from intercropped peanut roots (b), monocropped peanut roots (c), and monocropped peanut roots with control DMA and NA (d). Retention time (15.4–15.6 min). The arrows indicate the peaks of derivatized DMA ([di-FMOC-DMA+H], 527 and 749).
Figure S5. Phylogenetic analysis of yellow stripe-like proteins (YSL) ZmYS1, HvYS1, Arabidopsis AtYSLs, rice OsYSLs, and peanut AhYSLs. The peanut YSLs were named according to the nearby Arabidopsis YSLs. Accession numbers are as follows: AhYSL1, JQ248576; AhYSL3.1, JQ248577; AhYSL3.2, JQ248578; AhYSL4, GO264375; AhYSL6, JQ248579; AtYSL1, At4g24120; AtYSL2, At5g24380; AtYSL3, At5g53550; AtYSL4, At5g41000; AtYSL5, At3g17650; AtYSL6, At3g27020; AtYSL7, At1g65730; AtYSL8, At1g48370; ZmYS1, AF186234; HvYS1, AB214183; OsYSL1, AB190912; OsYSL2, AB126253; OsYSL3, AB190913; OsYSL4, AB190914; OsYSL5, AB190915; OsYSL6, AB190916; OsYSL7, AB190917; OsYSL8, AB190918; OsYSL9, AB190919; OsYSL10, AB190920; OsYSL11, AB190921; OsYSL12, AB190922; OsYSL13, AB164644; OsYSL14, AB164645; OsYSL15, AB190923; OsYSL16, AB190924; OsYSL17, AB190925; OsYSL18, AB190926.
Figure S6. Sequence alignment among AhYSL1, AhYSL3.1, and ZmYS1 proteins.
Table S1. Primers used in this study.
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