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pce12144-sup-0001-si.doc15392K

Figure S1. Characterization of cat2-1 mutant grown under low light conditions (30 μmol m−2 s−1). (a–c) Top, 3-week-old wild-type, cat2-1 and cat2-1 CAT2::CAT2 plants grown under 30 μmol m−2 s−1 light in LD conditions. Bars = 5 mm. (a–c) Bottom, transverse sections through leaves of the corresponding plants. Bars = 1 mm. (d) H2O2 content in the leaves of 3-week-old wild-type, cat2-1 and cat2-1 CAT2::CAT2 plants grown at 30 μmol m−2 s−1. Means and SD were calculated from three independent experiments. FW, fresh weight.

Figure S2. The changes of leaf phenotype and H2O2 contents of both wild-type and cat2-1 plants exposed to the light of 150 μmol m−2 s−1 after growth under low light. Three-week-old wild-type and cat2-1 plants were grown under low light (30 μmol m−2 s−1) in LD and subsequently transferred to the light of 150 μmol m−2 s−1 in LD conditions. The leaf phenotype and H2O2 contents were shown at 1 (a,b), 2 (c,d) and 3 d (e,f), respectively, after transferring. Top bars = 5 mm and bottom bars = 1 mm. Means and SD were calculated from three independent experiments. Significant differences are indicated by ***, P < 0.001. FW, fresh weight.

Figure S3. Characterization of plants transferred from moderate-intensity to low-intensity light. (a, b) Top, 3-week-old wild-type and cat2-1 plants were grown under the light of 150 μmol m−2 s−1 in LD and subsequently transferred to low light (30 μmol m−2 s−1) in LD conditions for 4 d. Bars = 5 mm. (a, b) Bottom, transverse sections through leaves of the corresponding plants. Bars = 1 mm. DR5::GUS (d) and cat2-1 DR5::GUS (e) plants, grown under the same conditions as described in (a) and (b), were stained for GUS activity. Top bars = 7 mm and bottom bars = 1 mm. H2O2 content (c) and IAA level (f) were measured in leaves of plants grown under the same conditions described in (a) and (b). Means and SD were calculated from three independent experiments. FW, fresh weight.

Figure S4. The changes of leaf phenotype and H2O2 contents of both wild-type and cat2-1 plants transferred from moderate-intensity to low-intensity light. Three-week-old wild-type and cat2-1 plants were grown under the light of 150 μmol m−2 s−1 in LD and subsequently transferred to low light (30 μmol m−2 s−1) in LD conditions. The leaf phenotype and H2O2 contents were shown at 1 (a,b), 2 (c,d) and 3 d (e,f), respectively, after transferring. Top bars = 5 mm and bottom bars = 1 mm. Means and SD were calculated from three independent experiments. FW, fresh weight.

Figure S5. GUS activity in DR5::GUS, cat2-1 DR5::GUS and cat2-1 DR5::GUS CAT2::iaaM lines. Three-week-old DR5::GUS, cat2-1 DR5::GUS and cat2-1 CAT2::iaaM DR5::GUS plants grown under moderate-intensity light (150 μmol m−2 s−1) and LD conditions were subjected to GUS staining. Bars = 5 mm.

Figure S6. Putative IAA biosynthesis pathways in Arabidopsis. Putative IAA biosynthesis pathways in Arabidopsis with genes that are down-regulated in cat2-1 plants grown under moderate-intensity light (150 μmol m−2 s−1) and LD conditions marked in blue. A dotted arrow indicates that there is more than one enzymatic step involved in the pathway.

Figure S7. qRT-PCR analysis of auxin biosynthesis-related genes. The expression of auxin biosynthesis-related genes was analysed by qPCR assays in the leaves of 3-week-old cat2-1 and wild-type plants grown under low-intensity light (30 μmol m−2 s−1) and LD conditions. The transcript levels of target genes were normalized to the expression of PDF2 (PROTEIN PHOSPHATASE 2A SUBUNIT A3, AT1G13320). All experiments were performed with three independent biological replicates and three technical repetitions. Error bars indicate the standard error of mean (SEM; N = 3). (a) shows transcript levels for the Trp biosynthetic genes, and (b) shows the expression of genes involved in Trp-dependent IAA biosynthesis. Significant differences to the wild type are indicated by **P < 0.01. (a) Transcript levels of the Trp biosynthetic genes and (b) of genes involved in Trp-dependent IAA biosynthesis.

Figure S8. Glutathione levels of the leaves of wild-type, cat2-1 and cat2-1 CAT2::iaaM plants under different light conditions. The glutathione levels were assayed in the leaves of 3-week-old wild-type, cat2-1 or cat2-1 CAT2::iaaM plants grown under either low light (a) or moderate light (b) in LD. The means and SD were calculated from three independent experiments.

Table S1. List of the primers used in qPCR analysis.

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