Figure S1. Venn diagram comparison between the data published by Osuna et al. (2007) and this work.
Figure S2. PageMan visualization of Arabidopsis gene expression changes in response to C depletion.
Figure S3. The expression pattern of C depletion-responding genes in Arabidopsis seedlings from various MapMan BINs.
Figure S4. Hierarchical image of the gene ontology (GO) terms associated with biological processes over-represented in genes that are induced in response to (a) 3, 6 or 12 h, (b) 24 h and (c) 48–72 h C depletion and repressed in response to (d) 3, 6 or 12 h, (e) 24 h and (e) 48–72 h C depletion.
Figure S5. Web interface displays from Usadel et al. (2008) showing the response of the genes in each cluster in Fig. to light, to C and to the circadian clock as well as the response during a diurnal cycle and extension of the night in Col-0 and a diurnal cycle in pgm.
Figure S6. Box plots of the gene expression responses of the genes that are (a) up-regulated and (b) down-regulated in response to 30 min sucrose readdition but do not respond to C depletion.
Figure S7. Relationship between the transcriptional response to sucrose readdition to Arabidopsis seedlings at different times in the C-depletion time series.
Figure S8. Comparison of the transcriptional responses during C depletion and sucrose readdition with the published response of overexpression of three C-signalling components: AKIN10 (SnRK1), bZIP11 and otsA (to increase Tre6P).
Table S1. The normalized signal intensities for all 22750 ATH1 probe sets from the 35 arrays from the C-depletion time course and cordycepin treatment experiments.
Table S2. PageMan input file with ATH1 identifier codes and gene expression changes during the C-depletion time course.
Table S3. Clustering of C-depletion responses.
Table S4. Analysis of MapMan BINs/functional groups over-represented in clusters of genes responding to C depletion.
Table S5. Transcripts responding to 30 min of 15 mm sucrose readdition to carbohydrate-starved seedlings.
Table S6. Analysis of functional groups over-represented in the 104 transcripts responding to sucrose readdition and to C depletion.
Table S7. Analysis of functional groups over-represented in the 46 transcripts responding to sucrose readdition and not responding to C depletion.
Table S8. Transcripts differentially expressed in response to 30 min of 15 mm sucrose readdition to C-depleted seedlings during the first 24 h of the time course.
Table S9. Transcripts differentially expressed in response to 30 min of 15 mm sucrose readdition or 1 h of 0.6 mm cordycepin A treatment to C-depleted seedlings.
Table S10. Analysis of functional groups over-represented in the transcripts induced by C depletion and repressed by 1 h of 0.6 mm cordycepin A treatment to C-depleted seedlings.
Table S11. Analysis of functional groups over-represented in the transcripts repressed by C depletion and 1 h of 0.6 mm cordycepin A treatment to C-depleted seedlings.
Table S12. Analysis of the promoter motifs over-represented in clusters of genes responding to C depletion.
Table S13. Analysis of the promoter motifs over-represented in genes repressed in response to 30 min of 15 mm sucrose readdition to C-depleted seedlings.
Table S14. Comparison of the transcriptional responses during C depletion and sucrose readdition with the published response of overexpression of three C-signalling components: AKIN10, bZIP11 and otsA.
Text S1. Explanation for Fig. S5.
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