Cold stress selectively unsilences tandem repeats in heterochromatin associated with accumulation of H3K9ac (pages 2130–2142)
YONG HU, LU ZHANG, SHIBIN HE, MIN HUANG, JUNJUN TAN, LIN ZHAO, SHIHAN YAN, HUI LI, KUN ZHOU, YANAN LIANG and LIJIA LI
Version of Record online: 19 JUN 2012 | DOI: 10.1111/j.1365-3040.2012.02541.x
It is not clear whether any type of heterochromatic elements can be activated by environmental stress and little is known about global epigenetic modification trends of repetitive sequences in large-genome plants in response to environmental stress. In this study, we identified differential cold-induced H3K9ac enrichment at repetitive sequences in maize by using a chromatin immunoprecipitation sequencing (ChIP-Seq) method and confirmed that cold stress unsilenced two knob-associated tandem repetitive sequences, 180-bp and TR-1, companied with elevated H3K9ac levels, decreased DNA methylation and H3K9me2 and nucleosome remodeling at the tandem repeat region. These results suggest that heterochromatic tandem repeated sequences may play an important role in the adaptation of plants to cold stimuli.