RNAseq-based transcriptome analysis of Lactuca sativa infected by the fungal necrotroph Botrytis cinerea (pages 1992–2007)
KAAT DE CREMER, JANICK MATHYS, CHRISTINE VOS, LUTZ FROENICKE, RICHARD W. MICHELMORE, BRUNO P A. CAMMUE and BARBARA DE CONINCK
Article first published online: 26 APR 2013 | DOI: 10.1111/pce.12106
This study includes an extended RNAseq analysis of the locally induced defence responses in leaves of lettuce after inoculation with Botrytis cinerea, an economically important pathogen affecting many dicot plant species. To our knowledge, this is the first reported quantitative transcriptomic study of lettuce, which was possible after the recent unravelling of the lettuce genome. The observed high number of differentially expressed genes allowed us to classify them according to the biological pathways indicating the induction of secondary metabolism, with the phenylpropanoid pathway and terpenoid biosynthesis among the most pronounced responses. Subsequent qRT-PCR-based gene expression analysis on a limited set of genes resulting from our RNAseq-study revealed a high similarity in (i) the response in locally infected versus systemic leaves and (ii) in the induction of similar pathways during compatible interactions of lettuce with necrotrophic versus biotrophic pathogens.