These authors contributed equally to this work.
Genome-wide promoter methylation analysis identifies epigenetic silencing of MAPK13 in primary cutaneous melanoma
Article first published online: 17 APR 2013
© 2013 John Wiley & Sons A/S
Pigment Cell & Melanoma Research
Volume 26, Issue 4, pages 542–554, July 2013
How to Cite
Gao, L., Smit, M. A., van den Oord, J. J., Goeman, J. J., Verdegaal, E. M. E., van der Burg, S. H., Stas, M., Beck, S., Gruis, N. A., Tensen, C. P., Willemze, R., Peeper, D. S. and van Doorn, R. (2013), Genome-wide promoter methylation analysis identifies epigenetic silencing of MAPK13 in primary cutaneous melanoma. Pigment Cell & Melanoma Research, 26: 542–554. doi: 10.1111/pcmr.12096
- Issue published online: 26 JUN 2013
- Article first published online: 17 APR 2013
- Accepted manuscript online: 21 MAR 2013 11:13AM EST
- Manuscript Accepted: 11 MAR 2013
- Manuscript Received: 29 NOV 2012
- Dutch Cancer Society
|pcmr12096-sup-0001-FigureS1.eps||image/eps||3735K||Figure S1. CpG islands within gene promoter regions are analyzed for validation of gene methylation status.|
|pcmr12096-sup-0002-FigureS2.eps||image/eps||18295K||Figure S2. Promoter methylation status of HOXA9, MAPK13, CDH11, PLEKHG6, PPP1R3C, and CLDN11 in metastatic melanoma samples.|
|pcmr12096-sup-0003-FigureS3.eps||image/eps||4133K||Figure S3. Restoration of MAPK13 expression in melanoma cells with MAPK13 promoter methylation attenuates proliferation, long-term growth curves.|
|pcmr12096-sup-0004-FigureS4.eps||image/eps||2451K||Figure S4. Depletion of MAPK13 does not prevent cell cycle arrest induced by BRAFV600E in human fibroblasts.|
Table S1. (A) Fresh-frozen biopsies of nevus, primary cutaneous melanoma, and melanoma metastases; (B) Early passage melanoma cell lines, isolated from metastatic lesions of patients treated at Leiden University Medical Center.
Table S2. (A) Bisulfite primers; (B) qPCR primers; (C) BRAF and NRAS sequencing primers.
Table S3. (A) List of the 106 unique genes (P < 0.05 and average β-value difference > 0.20) identified as hypermethylated in primary melanoma. (B) List of the 44 unique genes (P < 0.05 and average β-value difference > −0.20) identified as hypomethylated in primary melanoma.
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