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pcmr12139-sup-0001-DataS1.docWord document33KData S1. Materials and Methods.
pcmr12139-sup-0002-FigureS1.tifimage/tif946KFigure S1. (A) Relative cell viability of WM164 cells treated with PLX4032 (1 μM), GSK1120212 (50 nM) or in combination of either with TGF-β1 (5 ng/ml), in comparison with DMSO- (control) and TGF-β1-treated cells. (B) Percentage of caspase-3-positive cells after exposure to PLX4032 (1 μM), GSK1120212 (50 nM) in combination with TGF-β1 (5 ng/ml) for 36 h in WM9 cells (***P-value <0.001- Student's t-test). (C) Percentage of caspase-3-positive cells after exposure to SB431542 (10 μM), PLX4032 (1 μM) alone or in combination with TGF-β1 for 36 h in WM164 cells. (D) Western blot of Snail1 and Slug in WM164 cells treated with PLX4032 (1 μM) or TGF-β1 (5 ng/ml) alone, or in combination of both, with beta actin as a loading control.
pcmr12139-sup-0003-FigureS2.tifimage/tif710KFigure S2. (A) Twist1 overexpression (WM35 Twist1) in WM35 cells reduces the induction of apoptosis to treatment with PLX4032 (1 μM) even in the presence of TGF-β1 (5 ng/ml) if compared with empty vector control (WM35 EV) (***P-value <0.001- two-way anova). (B) Immunoblot showing levels of Twist1 in WM164 EV, WM164 SH Twist1 and WM164 Twist1 overexpressing (WM164 Twist1 OV) cells before and after 12 days of PLX4032 (1 μM) treatment.
pcmr12139-sup-0004-FigureS3.tifimage/tif919KFigure S3. (A), (B) Percentage of caspase-3-positive cells in WM164 cells treated with TNF-α (10 ng/ml), TGF-β1 (5 ng/ml) and GSK1120212 (50 nM) alone or in combination in comparison with cells exposed to DMSO (control) (***P-value <0.001- Student's t-test). (C) Percentage of caspase-3-activated cells in WM164 cells treated with PLX4032 and the STAT3 inhibitor VII (1 μM) in comparison with a combination of the same with TNF-α (***P-value <0.001- Student's t-test). (D) Percentage of caspase-3-positive cells in SKMEL28 cells treated with TNF-α (10 ng/ml), TGF-β1 (5 ng/ml), PLX4032 (1 μM) or GSK1120212 (50 nM) alone or in combination (***P-value <0.001- Student's t-test).

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