Figure S1. Normal resting membrane potential of dali mutant melanophores.

Figure S2. (A)Alignment of Tspan3 proteins and schematic of Tspan3c.

Figure S3. Tspan3c-EGFP fusion protein is functional.

Figure S4. Difference between EGFP fluorescence and xanthophore autofluorescence in Tg(tspan3c:EGFP) F0 fish.

Figure S5.tspan3c promoter-driven EGFP expression in melanophore and iridophore.

Figure S6. Tspan3c(WT or I18R)-EGFP fusion proteins were expressed in B16 melanoma cells and their localization was examined using markers for ER (A) and Golgi apparatus (B).

pcmr12192-sup-0002-VideoS1.movvideo/mov9665KVideo S1. A wild-type melanophore actively migrates. ‘M’ indicates melanophore, ‘X’ indicates xanthophore, and scale bars indicate 100 μm in the supplementary videos.
pcmr12192-sup-0003-VideoS2.movvideo/mov9493KVideo S2. Both melanophores and xanthophores from the dali mutant fail to migrate.
pcmr12192-sup-0004-VideoS3.movvideo/mov10299KVideo S3. A wild-type xanthophore migrates only after contacting a wild-type melanophore. Melanophores migrate away from such contacts (WT-X 1 and WT-X 2). The melanophore near WT-X 3 did not show this repulsive behavior.
pcmr12192-sup-0005-VideoS4.movvideo/mov4246KVideo S4. A dali mutant melanophore and dali mutant xanthophore fail to exhibit ‘escape and chase’ behaviors following contact.
pcmr12192-sup-0006-VideoS5.movvideo/mov9358KVideo S5. A dali mutant melanophore is not stimulated to migrate even after contacting with a wild-type xanthophore.
pcmr12192-sup-0007-VideoS6.movvideo/mov8827KVideo S6. A wild-type melanophore does not migrate away after contact with a dali mutant xanthophore.

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