Red human hair pheomelanin is a potent pro-oxidant mediating UV-independent contributory mechanisms of melanomagenesis
Version of Record online: 6 JAN 2014
© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Pigment Cell & Melanoma Research
Volume 27, Issue 2, pages 244–252, March 2014
How to Cite
Panzella, L., Leone, L., Greco, G., Vitiello, G., D'Errico, G., Napolitano, A. and d'Ischia, M. (2014), Red human hair pheomelanin is a potent pro-oxidant mediating UV-independent contributory mechanisms of melanomagenesis. Pigment Cell & Melanoma Research, 27: 244–252. doi: 10.1111/pcmr.12199
- Issue online: 17 FEB 2014
- Version of Record online: 6 JAN 2014
- Accepted manuscript online: 30 NOV 2013 02:15PM EST
- Manuscript Accepted: 26 NOV 2013
- Manuscript Received: 19 SEP 2013
- Italian MIUR. Grant Number: PRIN 2010–2011-010PFLRJR
Figure S1. Residual (%) GSH after 3 h in 0.1 M phosphate buffer (pH 7.4) in the absence (control, n = 5) or in the presence of RHP (n = 5) or BHE (n = 5). (Initial substrate concentration: 2 mM; substrate/melanin=1:2 w/w). All values are expressed as the mean ± SD. Significant differences were determined by independent samples two-tailed t test. P < 0.05 is considered significant; exact P-values are shown in each plot.
Figure S2. Residual (%) GSH after 1 h (A), 2 h (B) or 3 h (C) in 0.1 M phosphate buffer (pH 7.4) in the presence of variable amounts of RHP (n = 5 for each concentration) (initial concentration of GSH: 2 mM). All values are expressed as the mean ± SD. Trend lines and correlation coefficients are indicated.
Figure S3. Residual (%) GSH after 1 h in 0.1 M phosphate buffer (pH 7.4) under different reaction conditions as indicated in the Methods section. All values are expressed as the mean ± SD (control, n = 7; other conditions, n = 5).
Figure S4. EPR spectra of CD-mel (A), RHP (B) and BHE (C). The signal at low magnetic field is due to iron ions. The intermediate signals indicate the presence of copper(II) ions. The singlet signal is from the melanin stable radical (g = 2.005). The presence of iron ions in the CD-mel sample is likely due to contaminants from the phosphate buffer used for melanin preparation.
Figure S5. EPR spectrum of RHP [0.5 mg/ml 0.1 M phosphate buffer (pH 7.4)] before (A) and after (B) 24 h incubation in the absence of GSH.
Figure S6. EPR spectrum of CD-mel [0.5 mg/ml 0.1 M phosphate buffer (pH 7.4)] before (A) and after 24 h incubation in the absence (B) or in the presence (C) of GSH (1: 5 w/w).
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.