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Resistance of Drosophila suzukii to the larval parasitoids Leptopilina heterotoma and Asobara japonica is related to haemocyte load

Authors

  • MATHILDE POYET,

    1. Unité Ecologie et Dynamique des Systémes Anthropisés, Université de Picardie Jules Verne, Amiens, France
    2. Laboratoire de Biométrie et Biologie Evolutive, Université Lyon, Lyon 1, France
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  • SEBASTIEN HAVARD,

    1. Laboratoire Diversité, Génomes et Interactions Microorganismes-Insectes, Université de Montpellier 2, Montpellier, France
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  • GENEVIEVE PREVOST,

    1. Unité Ecologie et Dynamique des Systémes Anthropisés, Université de Picardie Jules Verne, Amiens, France
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  • OLIVIER CHABRERIE,

    1. Unité Ecologie et Dynamique des Systémes Anthropisés, Université de Picardie Jules Verne, Amiens, France
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  • GERALDINE DOURY,

    1. Unité Ecologie et Dynamique des Systémes Anthropisés, Université de Picardie Jules Verne, Amiens, France
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  • PATRICIA GIBERT,

    1. Laboratoire de Biométrie et Biologie Evolutive, Université Lyon, Lyon 1, France
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  • PATRICE ESLIN

    Corresponding author
    1. Unité Ecologie et Dynamique des Systémes Anthropisés, Université de Picardie Jules Verne, Amiens, France
      Patrice Eslin, Laboratoire de Bio-écologie des Insectes Phytophages et Entomophages, Université de Picardie Jules Verne, 33 rue Saint Leu, F-80039 Amiens Cedex, France. Tel.: +33 (0)3 22 82 75 47; e-mail: patrice.eslin@u-picardie.fr
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Patrice Eslin, Laboratoire de Bio-écologie des Insectes Phytophages et Entomophages, Université de Picardie Jules Verne, 33 rue Saint Leu, F-80039 Amiens Cedex, France. Tel.: +33 (0)3 22 82 75 47; e-mail: patrice.eslin@u-picardie.fr

Abstract

Unlike other Drosophila species, the invasive Drosophila suzukii Matsumura (Diptera: Drosophilidae) shows a remarkable pest status. Among the physiological traits that may explain the high level of resistance to parasitoids of Drosophila larvae, the haemocyte load is shown repeatedly to play an important role. To determine whether haemocyte load can explain immunity resistance of D. suzukii to parasitoids, the haemocytes of parasitized and healthy larvae are quantified in two Japanese and three French populations of D. suzukii. Parasitization tests are conducted with two larval parasitoids: the paleartic Leptopilina heterotoma Thomson (Hymenoptera: Figitidae) and the Asian Asobara japonica Belokobylskij (Hymenoptera: Braconidae). Based on morphological and functional criteria, D. suzukii has classes of haemocytes similar to those described in Drosophila melanogaster. However, healthy larvae of the five populations tested possess particularly large numbers of haemocytes compared with D. melanogaster. Haemocyte load is also higher in larvae from the French populations than in the Japanese strains. The ability of D. suzukii larvae to encapsulate eggs of L. heterotoma is associated with a particularly high load of circulating haemocytes. However, it is notable that A. japonica induces a strong depression of the haemocyte population in this resistant host associated with an inability to encapsulate parasitoid eggs. The results show that the cellular immune system plays a major role in the failure of larval parasitoids to develop in most instances in larvae of D. suzukii, possibly contributing to the success of this species as an invader.

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