php12004-sup-0001-FigS1.pdfapplication/PDF31KFigure S1. SDS-PAGE gel of the LM-LOV-STAS eluted with 150 mm imidazole (Lane 7). It contains mostly monomer and a small amount of dimer. Lanes 1 and 2 are prestained protein molecular weight markers—SeeBlue Plus2 Pre-Stained Standard (Invitrogen) and ColorBurst Electrophoresis Marker (Sigma Aldrich), respectively. Lane 3 is the lysate after French-pressing E. coli. Lane 4 is the column flow through. Lanes 5 and 6 are the equilibration buffer wash and 20 mm imidazole wash, respectively.

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