Multiplexed, UVC-Induced, Sequence-Dependent DNA Damage Detection
Article first published online: 22 MAR 2013
© 2013 Wiley Periodicals, Inc. Photochemistry and Photobiology © 2013 The American Society of Photobiology
Photochemistry and Photobiology
Special Issue: Symposium in Print on Upper-Room Ultraviolet Germicidal Irradiation for Air Disinfection
Volume 89, Issue 4, pages 884–890, July/August 2013
How to Cite
Nair, S. G. and Loppnow, G. R. (2013), Multiplexed, UVC-Induced, Sequence-Dependent DNA Damage Detection. Photochemistry and Photobiology, 89: 884–890. doi: 10.1111/php.12066
- Issue published online: 2 JUL 2013
- Article first published online: 22 MAR 2013
- Accepted manuscript online: 27 FEB 2013 05:53AM EST
- Manuscript Accepted: 22 FEB 2013
- Manuscript Received: 13 NOV 2012
- Natural Science and Engineering Research Council of Canada
- Alberta Cancer Board
Figure S1. Melting (filled squares) and cooling curve (open circles) of 0.18 µm SPTarC alone. The melting curve was generated at a heating rate of 1°C min−1, in 4°C increments and with a 5 min holding time after each increment. The cooling curve was performed with all the above conditions, except −4°C increments were used.
Figure S2. Absorbance at 260 nm as a function of UVC exposure time for a 10 µm dT17 irradiated sample (filled squares) and 10 µm unirradiated dT17 control (open circles).
Figure S3. Fluorescence intensities for different ratios of SPTarC:TarC. Different ratios are obtained by keeping the concentration of SP constant at 0.18 µm and varying the concentration of target.
Figure S4. Melting curves of 0.18 µm SP alone (open circles) and 0.18 µm SP in the presence of a three-fold excess of perfectly complementary oligonucleotide target sequence (filled squares) in 10 mm Tris and 1 mm EDTA with varying Na+ and Mg2+ concentrations. The melting curves use (A) 1 mm MgCl2, (B) 3 mm MgCl2, (C) 3 mm MgCl2 and 20 mm NaCl and (D) 5 mm MgCl2 and 20 mm NaCl. Fluorescence curves have each been scaled to SP alone.
Figure S5. Moles of triiodide formed as a function of exposure time for (A) Cuvette method and (B) Well plate method. The solid line through the points are linear fit with slope of the calibration curve for cuvette method = 1.31 × 10−10 mol s−1 and well plate method = 4.01 × 10−12 mol s−1 respectively.
Table S1. Fluorescence intensity of blank sample.
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