Tryptophan Cluster Protects Human γD-Crystallin from Ultraviolet Radiation-Induced Photoaggregation In Vitro
Article first published online: 20 JUN 2013
© 2013 The Authors. Photochemistry and Photobiology published by Wiley Periodicals, Inc. on behalf of The American Society of Photobiology.
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Photochemistry and Photobiology
Volume 89, Issue 5, pages 1106–1115, September/October 2013
How to Cite
Schafheimer, N. and King, J. (2013), Tryptophan Cluster Protects Human γD-Crystallin from Ultraviolet Radiation-Induced Photoaggregation In Vitro. Photochemistry and Photobiology, 89: 1106–1115. doi: 10.1111/php.12096
- Issue published online: 6 SEP 2013
- Article first published online: 20 JUN 2013
- Accepted manuscript online: 20 MAY 2013 05:05AM EST
- Manuscript Accepted: 12 MAY 2013
- Manuscript Received: 15 FEB 2013
- National Eye Institute. Grant Number: #EY015834
- NSF. Grant Number: 007031
|php12096-sup-0001-FigureS1.tif||image/tif||3081K||Figure S1. (a) Changes in solution turbidity measuring OD600 (red triangles), OD350 (green diamonds) and OD280 (blue squares) over UVR exposure time. Samples contained 0.25 mg mL−1 WT HγD-Crys in sample buffer. (b) Lag time to turbidity development versus concentrations of WT HγD-Crys observed via OD600 (red triangles), OD350 (green diamonds) and OD280 (blue squares). Turbidity data were fit to sigmoidal curves and the time to increase to 0.05 au above baseline OD at time 0 was calculated as the lag time.|
|php12096-sup-0002-FigureS2.tif||image/tif||2246K||Figure S2. Changes in solution turbidity measuring OD600 of samples at pH 4 (blue diamonds), pH 5 (red squares), pH 6 (green triangles), pH 7 (purple X's), pH 8 (turquoise hatches) and pH 8.8 (orange circles) over UVR exposure time. Samples contained 0.25 mg mL−1 WT HγD-Crys in sample buffer. Solutions at pH 4 and 5 were produced by adding concentrated HCl to sample buffer beforehand. Solutions pH's were determined using a Thermo Orion PerpHect LogR Meter (model 330) with 8156BNUWP probe.|
|php12096-sup-0003-FigureS3.tif||image/tif||2004K||Figure S3. Changes in solution turbidity of 0.25 mg mL−1 WT HγD-Crys samples, measuring OD600, of samples at 4°C (blue diamonds), 16°C (red squares), 25°C (green triangles) and 37°C (purple X's) over UVR exposure time.|
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