This article is part of the Special Issue dedicated to the memory of Elsa Abuin.
Impact of Dye-Protein Interaction and Silver Nanoparticles on Rose Bengal Photophysical Behavior and Protein Photocrosslinking†
Article first published online: 17 AUG 2013
© 2013 The American Society of Photobiology
Photochemistry and Photobiology
Volume 89, Issue 6, pages 1433–1441, November/December 2013
How to Cite
Simpson, M. J., Poblete, H., Griffith, M., Alarcon, E. I. and Scaiano, J. C. (2013), Impact of Dye-Protein Interaction and Silver Nanoparticles on Rose Bengal Photophysical Behavior and Protein Photocrosslinking. Photochemistry and Photobiology, 89: 1433–1441. doi: 10.1111/php.12119
- Issue published online: 4 NOV 2013
- Article first published online: 17 AUG 2013
- Accepted manuscript online: 26 JUN 2013 01:25AM EST
- Manuscript Accepted: 18 JUN 2013
- Manuscript Received: 26 APR 2013
The role of recombinant Type-I human collagen in the free form or forming AgNP@collagen on the photophysical and photochemical behavior of rose Bengal was analyzed. The formation of dye aggregates on the protein surface was experimentally observed and corroborated by docking calculations. The formation of such aggregates is believed to change the main oxidative mechanism from Type-II (singlet oxygen) to Type-I (free radical) photosensitization. Remarkably, the presence of AgNP in the form of AgNP@collagen altered the dynamics of dye triplet deactivation, effectively preventing the dye degradation and reducing the extent of protein crosslinked. Both crosslinked rHC and AgNP@collagen were able to support fibroblasts proliferation, but only the material containing silver was resistant to S. epidermidis infection.