This article is part of the Special Issue dedicated to the memory of Elsa Abuin.
Association of Valdecoxib, a Nonsteroidal Anti-Inflammatory Drug, with Human Serum Albumin†
Article first published online: 16 SEP 2013
© 2013 The American Society of Photobiology
Photochemistry and Photobiology
Volume 89, Issue 6, pages 1399–1405, November/December 2013
How to Cite
Encinas, M. V., Lissi, E. and Vergara, C. (2013), Association of Valdecoxib, a Nonsteroidal Anti-Inflammatory Drug, with Human Serum Albumin. Photochemistry and Photobiology, 89: 1399–1405. doi: 10.1111/php.12158
- Issue published online: 4 NOV 2013
- Article first published online: 16 SEP 2013
- Accepted manuscript online: 16 AUG 2013 12:09PM EST
- Manuscript Accepted: 12 AUG 2013
- Manuscript Received: 3 APR 2013
- FONDECYT. Grant Number: 1110536
Valdecoxib addition quenches the intrinsic human serum albumin (HSA) fluorescence. This allows an evaluation of the drug–protein association. However, both the number of binding sites and their affinity for the drug depend upon the methodology employed for their evaluation and the employed protein concentration. In this work, we measured the effect of valdecoxib on HSA fluorescence yield over a wide range of experimental conditions and discuss the validity of the binding parameters derived from the different data treatments: Stern–Volmer, Scatchard, double logarithmic, quadratic equation, Benesi–Hilderand, and Encinas–Lissi. It is proposed that a combination of Encinas–Lissi and Scatchard treatments of the data renders the most reliable results. From these data, it is concluded that HSA presents three high-affinity binding sites for valdecoxib (Kas = 4.5 × 104 m−1) and several secondary sites of smaller activity.