PDT Dose Parameters Impact Tumoricidal Durability and Cell Death Pathways in a 3D Ovarian Cancer Model (pages 942–952)
Imran Rizvi, Sriram Anbil, Nermina Alagic, Jonathan P. Celli, Lei Zak Zheng, Akilan Palanisami, Michael D. Glidden, Brian W. Pogue and Tayyaba Hasan
Version of Record online: 4 APR 2013 | DOI: 10.1111/php.12065
The photosensitizer-light parameters used to construct a photodynamic therapy (PDT) dose significantly impact tumor destruction and regrowth kinetics. A 3D ovarian cancer model (a) is used to evaluate a matrix of benzoporphyin derivative (BPD) and light combinations for three fixed PDT doses (b). PDT with 0.25 μM BPD (blue) provides maximum cytotoxic durability for all doses (c). Representative LIVE/DEAD (green/red) images (d) that were used to generate normalized viabilities in (c), depict increased killing in nodules treated with 0.25 μM BPD-PDT (2) compared to PDT with 1 μM (3) or 10 μM (4) BPD. These findings suggest that customization of PDT parameters could improve treatment outcomes.