Conflicts of interest:
Antiphotoaging effect of chitooligosaccharides on human dermal fibroblasts
Article first published online: 6 NOV 2012
© 2012 John Wiley & Sons A/S
Photodermatology, Photoimmunology & Photomedicine
Volume 28, Issue 6, pages 299–306, December 2012
How to Cite
Kim, J.-A., Ahn, B.-N., Kong, C.-S., Park, S.-H., Park, B.-J. and Kim, S.-K. (2012), Antiphotoaging effect of chitooligosaccharides on human dermal fibroblasts. Photodermatology, Photoimmunology & Photomedicine, 28: 299–306. doi: 10.1111/phpp.12004
- Issue published online: 6 NOV 2012
- Article first published online: 6 NOV 2012
- Manuscript Accepted: 1 AUG 2012
- Marine Bioprocess Research Center of the Marine Biotechnology Program
- Ministry of Land, Transport and Maritime
- 3–5 kDa chitooligosaccharide;
- collagen-degrading MMPs;
In the present study, the effect of 3–5 kDa chitooligosaccharide (COS) on homeostasis between the expression of collagen-degrading matrix metalloproteinases (MMPs) and collagen synthesis was investigated using ultraviolet (UV)-A irradiated dermal fibroblasts.
UV protection imparted by 3–5 kDa COS was measured by examining the UV absorption spectrum. Collagenase MMP secretion was examined using an enzyme-linked immunosorbent assay. The levels of collagenases and collagen synthetic markers were determined by employing the reverse transcriptase-polymerase chain reaction and Western blot analysis.
The 3–5 kDa COS not only absorbed UV-A and UV-B light but also inhibited collagenase (MMP-1, MMP-8, and MMP-13) and gelatinase (MMP-2 and MMP-9) MMP expression. The suppression of MMP expression was found to be due to an increase in expression of the tissue inhibitors of MMP (TIMP)-1 and TIMP-2. Treatment with 3–5 kDa COS enhanced collagen synthetic markers such as procollagen, type I, III, and IV collagens in UV-A-irradiated dermal fibroblasts. Furthermore, the effects of 3–5 kDa COS on collagen degradation and collagen synthesis in UV-A irradiated dermal fibroblasts were regulated via the inhibition of activating protein-1 (AP-1) signaling.
Our results suggest that 3–5 kDa COS can be used to develop as topical applications for antiphotoaging cosmeceuticals as it enhances collagen synthesis.