Increased endocytosis of fluorescent phospholipid in tobacco pollen in microgravity and inhibition by verapamil
Article first published online: 25 JUL 2013
© 2013 German Botanical Society and The Royal Botanical Society of the Netherlands
Special Issue: Plant Biology in Space. Guest Editors: V. Legué & K. Palme. The German Botanical Society, the Royal Botanical Society of the Netherlands and Wiley have published this supplement without financial support.
Volume 16, Issue Supplement s1, pages 107–112, January 2014
How to Cite
Scherer, G. F. E., Quader, H. (2014), Increased endocytosis of fluorescent phospholipid in tobacco pollen in microgravity and inhibition by verapamil. Plant Biology, 16: 107–112. doi: 10.1111/plb.12061
- Issue published online: 23 DEC 2013
- Article first published online: 25 JUL 2013
- Manuscript Accepted: 17 MAY 2013
- Manuscript Received: 31 JAN 2013
- Bundesministerium für Forschung. Grant Numbers: 50WB0010, 50WB0033
- tobacco pollen
Gravity sensing in plants occurs in specialised tissues, like in the columella in root tips or the endodermis in shoots. Generally, dense organelles, acting as statoliths, are thought to interact with the cytosekeleton and ion channels in gravitropism. We examined the possibility that tobacco pollen tubes (Nicotiana sylvestris) having an elaborate cytoskeleton could perceive gravity through interaction of the cytoskeleton and the endomembrane system and organelles. Using lipid endocytosis as a quantitative parameter, we show that endocytosis is increased transiently in microgravity within 3 min. This increase is inhibited by the calcium blocker verapamil, suggesting that calcium is lowered in the tip, which is known to increase endocytosis in the pollen tube.