The metabolic response of cultured tomato cells to low oxygen stress

Authors

  • J. Ampofo-Asiama,

    1. Division of Mechatronics, Department of Biosystems (BIOSYST), Biostatistics and Sensors (MeBioS), KU Leuven, Leuven, Belgium
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  • V. M. M. Baiye,

    1. Division of Mechatronics, Department of Biosystems (BIOSYST), Biostatistics and Sensors (MeBioS), KU Leuven, Leuven, Belgium
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  • M. L. A. T. M. Hertog,

    Corresponding author
    1. Division of Mechatronics, Department of Biosystems (BIOSYST), Biostatistics and Sensors (MeBioS), KU Leuven, Leuven, Belgium
    • Correspondence

      M. L. A. T. M. Hertog, Division of Mechatronics, Biostatistics and Sensors (MeBioS), Department of Biosystems (BIOSYST), KU Leuven, Willem de Croylaan 42, 3001 Leuven, Belgium.

      E-mail: maarten.hertog@biw.kuleuven.be

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  • E. Waelkens,

    1. Department of Cellular and Molecular Medicine, KU Leuven, Leuven, Belgium
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  • A. H. Geeraerd,

    1. Division of Mechatronics, Department of Biosystems (BIOSYST), Biostatistics and Sensors (MeBioS), KU Leuven, Leuven, Belgium
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  • B. M. Nicolai

    1. Division of Mechatronics, Department of Biosystems (BIOSYST), Biostatistics and Sensors (MeBioS), KU Leuven, Leuven, Belgium
    2. Flanders Centre of Postharvest Technology (VCBT), Leuven, Belgium
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Abstract

The storage of fruits and vegetables under a controlled atmosphere can induce low oxygen stress, which can lead to post-harvest losses through the induction of disorders such as core breakdown and browning. To gain better understanding of the metabolic response of plant organs to low oxygen, cultured tomato cells (Lycopersicum esculentum) were used as a model system to study the metabolic stress response to low oxygen (0 and 1 kPa O2). By adding 13C labelled glucose, changes in the levels of polar metabolites and their 13C label accumulation were quantified. Low oxygen stress altered the metabolite profile of tomato cells, with the accumulation of the intermediates of glycolysis in addition to increases in lactate and sugar alcohols. 13C label data showed reduced label accumulation in almost all metabolites except lactate and some sugar alcohols. The results showed that low oxygen stress in tomato cell culture activated fermentative metabolism and sugar alcohol synthesis while inhibiting the activity of the TCA cycle and the biosynthesis of metabolites whose precursors are derived from central metabolism, including fluxes to most organic acids, amino acids and sugars.

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