The miRNA and mRNA Changes in Rat Hippocampi after Chronic Constriction Injury

Authors

  • Masae Arai MD,

    Corresponding author
    • Department of Anesthesiology and Pain Medicine, Graduate School of Nippon Medical School, Tokyo, Japan
    Search for more papers by this author
    • study concept and design, acquisition of data, analysis and interpretation of data,
    • drafting the manuscript for important intellectual content,
    • approval of the final version to be published.
  • Yuuki Genda MD,

    1. Department of Anesthesiology and Pain Medicine, Graduate School of Nippon Medical School, Tokyo, Japan
    Search for more papers by this author
    • study concept and design, acquisition of data, analysis and interpretation of data,
  • Masashi Ishikawa MD,

    1. Department of Anesthesiology and Pain Medicine, Graduate School of Nippon Medical School, Tokyo, Japan
    Search for more papers by this author
    • study concept and design, acquisition of data, analysis and interpretation of data,
  • Tanaka Shunsuke MD,

    1. Department of Anesthesiology and Pain Medicine, Graduate School of Nippon Medical School, Tokyo, Japan
    Search for more papers by this author
    • study concept and design, acquisition of data, analysis and interpretation of data,
  • Tadashi Okabe PhD,

    1. Department of Anesthesiology and Pain Medicine, Graduate School of Nippon Medical School, Tokyo, Japan
    Search for more papers by this author
    • study concept and design, acquisition of data, analysis and interpretation of data,
  • Atsuhiro Sakamoto MD, PhD, Prof.

    1. Department of Anesthesiology and Pain Medicine, Graduate School of Nippon Medical School, Tokyo, Japan
    Search for more papers by this author
    • drafting the manuscript for important intellectual content,
    • approval of the final version to be published.

  • Contributions:

Reprint requests to: Masae Arai, MD, Department of Anesthesiology and Pain Medicine, Graduate School of Nippon Medical, School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan. Tel: +81-3-5814-6243; Fax: +81-3-5685-3077; E-mail: masae-a@nms.ac.jp.

Abstract

Objective

We elaborated the rat hippocampi in order to assess for central nervous system changes following a peripheral neuropathic injury.

Design, SettingSubjects

We examined the gene changes in the hippocampi of chronic constriction injury (CCI) rats with TaqMan® low-density array analysis (TLDA) and quantitative real-time polymerase chain reaction (qRT-PCR) of miR-125b, -132, and messenger RNAs (mRNAs) of neuropeptide Y, brain-derived neural factor, N-methyl-D-aspartate glutamate 2A receptor, gamma-aminobutyric acid A a1 receptor, gamma-aminobutyric acid A b1 receptor, gamma-aminobutyric acid B b2 receptor, serotonin 1A receptor, serotonin 2A receptor, serotonin 2C receptor, and serotonin 3A receptor on days 0, 7, and 15 after surgery.

Interventions

None.

Outcome Measures

Two behavioral tests (thermal and mechanical stimulation tests) were performed three times at 5-minute intervals to assess pain thresholds. MicroRNA (miRNA) changes were examined by TLDA. mRNA changes were examined by qRT-PCR. Statistical significance was determined by Tukey–Kramer's method and paired t-test.

Results

All rats showed mechanical and thermal hypersensitivity on the ipsilateral side. Out of 373 miRNAs analyzed, 237 were expressed, and 51 changed their expressions after CCI. By TLDA, cluster analysis found obvious miRNA changes on day 7 that tended to recover by day 15. For miR-125b, the relative expression decreased to 0.70 ± 0.30 at day 7 and recovered to 1.65 ± 0.19 at day 15. The miR-132 relative expressions were 0.69 ± 0.30 and 0.70 ± 0.15, respectively. The mRNA changes followed the miRNA changes.

Conclusions

Our results showed that the peripheral nerve injury altered rat hippocampal miRNA.

Ancillary