These authors contributed equally to this work.
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Original Article
Fast real-time detection of Potato spindle tuber viroid by RT-LAMP
Article first published online: 22 NOV 2012
DOI: 10.1111/ppa.12017
© 2012 British Society for Plant Pathology
Additional Information
How to Cite
Lenarčič, R., Morisset, D., Mehle, N. and Ravnikar, M. (2012), Fast real-time detection of Potato spindle tuber viroid by RT-LAMP. Plant Pathology. doi: 10.1111/ppa.12017
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These authors contributed equally to this work.
Publication History
- Article first published online: 22 NOV 2012
- Manuscript Accepted: 7 OCT 2012
Keywords:
- Isothermal amplification;
- melting curve;
- potato;
- PSTVd;
- RT-LAMP;
- viroid
This paper reports the development of a single tube, real-time, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting Potato spindle tuber viroid (PSTVd), one of the quarantine pathogens of potato in Europe and North America. The method enables detection of a broad range of PSTVd isolates, and is about 10 times more sensitive than the conventional reverse transcription polymerase chain reaction (RT-PCR) assay. Its benefits are not only its speed (15–25 min to obtain results) and cost effectiveness (resulting from time saved as well as cheaper consumables), but also its demonstrated ability to be performed in the field, using portable instruments.