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ppa12061-sup-0001-FigS1.tifimage/tif1658KFigure S1. The effect of temperature and wetness duration on the development of melanized appressoria of Colletotrichum acutatum on Parafilm-covered microscope slides. Shading transitions indicate increasing levels of melanized appressorium formation at 20% intervals starting at zero.
ppa12061-sup-0002-FigS2.tifimage/tif937KFigure S2. The effect of temperature and wetness duration on the incidence of infection of immature (a) and mature (b) blueberry fruits by Colletotrichum acutatum. Shading transitions indicate increasing levels of infected fruit at 20% intervals starting at zero.
ppa12061-sup-0003-FigS3.tifimage/tif741KFigure S3. The effect of interrupted wetness periods (0, 1, 4, and 16 h of dry time in the middle of 12, 24, and 48 h wetness periods) on the development of melanized appressoria (%) of Colletotrichum acutatum (a–c) and the percentage immature (d–f) and mature (g–i) blueberry fruit infected by C. acutatum. Values are the average of four replications and error bars denote the standard error of the mean.
ppa12061-sup-0004-FigS4.tifimage/tif1453KFigure S4. The effect of wetness duration and interrupted wetness periods on the development of melanized appressoria of Colletotrichum acutatum on Parafilm-covered microscope slides. Dry periods occurred in the middle of each wetness period in all experiments. Values are calculated as percentage of melanized appressoria relative to the continuous wetness control. Shading transitions indicate increasing levels of melanized appressoria at 20% intervals starting at zero.
ppa12061-sup-0005-FigS5.tifimage/tif931KFigure S5. The effect of wetness duration and interrupted wetness periods on the infection level of immature (a) and mature (b) blueberry fruits by Colletotrichum acutatum. Dry periods occurred in the middle of each wetness period in all experiments. Values are calculated as percentage of infected fruit relative to the continuous wetness control. Shading transitions indicate increasing levels of infected fruit at 20% intervals starting at zero.
ppa12061-sup-0006-FigS6.tifimage/tif1499KFigure S6. The effect of temperature and relative humidity on the development of melanized appressoria (%) of Colletotrichum acutatum (a), and the infection level of mature blueberry fruits (b) by C. acutatum. Shading transitions indicate increasing levels of melanized appressorium formation and infection of mature fruit at 20% intervals starting at zero.
ppa12061-sup-0007-TableS1.docxWord document24KTable S1. Statistics for fitted three-dimensional models of the percentage of melanized appressoria and infection incidence of immature and mature blueberry fruits by Colletotrichum acutatum in response to temperature (°C), wetness duration (h), wetness interruption (h) and relative humidity (%). Nine equations were chosen based on the best fit to the raw data from a mixture of experiments, and models were examined for goodness of fit (R2), standard error of the estimate (SEE), the P value of the regression and the mean squared error (MSE) of the residuals (* denotes a chosen equation).

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