Evidence of low levels of genetic diversity for the Phytophthora austrocedrae population in Patagonia, Argentina

Authors

  • M. L. Vélez,

    Corresponding author
    1. Protección Forestal, Centro de Investigación y Extensión Forestal Andino Patagónico (CIEFAP), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de la Patagonia San Juan Bosco (UNPSJB), Esquel, Argentina
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  • M. P. A. Coetzee,

    1. Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa
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  • M. J. Wingfield,

    1. Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa
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  • M. Rajchenberg,

    1. Protección Forestal, Centro de Investigación y Extensión Forestal Andino Patagónico (CIEFAP), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de la Patagonia San Juan Bosco (UNPSJB), Esquel, Argentina
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  • A. G. Greslebin

    1. Protección Forestal, Centro de Investigación y Extensión Forestal Andino Patagónico (CIEFAP), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de la Patagonia San Juan Bosco (UNPSJB), Esquel, Argentina
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Abstract

Phytophthora austrocedrae is a recently discovered pathogen that causes severe mortality of Austrocedrus chilensis in Patagonia. The high level of susceptibility of the host tree, together with the distribution pattern of the pathogen, have led to the hypothesis that P. austrocedrae was introduced into Argentina. The aim of this study was to assess the population structure of Paustrocedrae isolates from Argentina in order to gain an understanding of the origin and spread of the pathogen. Genetic diversity was determined based on amplified fragment length polymorphisms (AFLPs). In total, 48 isolates of Paustrocedrae were obtained from infected A. chilensis trees, representing the geographical range of the host. Four primer combinations were used for the AFLP analysis. Of the 332 scored bands, 12% were polymorphic. Gene diversity (h) ranged from 0·01 to 0·03; the Shannon index (I) ranged from 0·01 to 0·04. A high degree of genetic similarity was observed among the isolates (pairwise S values = 0·958–1; 0·993 ± 0·009, mean ± SD). A frequency histogram showed that most of the isolate pairs were identical. Principal coordinate analysis using three-dimensional plots did not group any of the isolates based on their geographical origin. The low genetic diversity (within and between sites) and absence of population structure linked to geographic origin, together with the aggressiveness of the pathogen and the disease progression pattern, suggest that Paustrocedrae might have been introduced into Argentina.

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