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ppa12077-sup-0001-FigureS1.tifimage/tif3969KFigure S1. Alternaria solani isolates grown on spiral-gradient end point dilution plates. (a) The boscalid sensitive isolate AS11 plated out on a boscalid-amended plate; (b) AS11 growing on a boscalid-free control plate (white arrows). Note there is very limited spore germination in the area where the spore suspension was applied to the surface of the medium in (a). (c) The boscalid resistant isolate AS4 growing on a boscalid-amended plate; (d) AS4 growing on a boscalid-free control plate (white arrows). Boscalid concentrations on the fungicide-amended plates ranged from 507 mg L−1 in the centre to 0·87 mg L−1 at the edge of the plates.
ppa12077-sup-0002-FigureS2.tifimage/tif2593KFigure S2. (a) Alignment of succinate dehydrogenase (SDH) subunit B gene sequences from a boscalid sensitive (AS11, wild type, WT) and a boscalid resistant (AS4) Alternaria solani isolate, indicating the differential restriction site for H277R. (b) Alignment of SDH subunit D gene sequences, indicating the differential restriction sites for H133R.

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